Microwave-assisted extraction and effect of Radix Rehmanniae Preparata on osteoblast in vitro

نویسندگان

  • Tianxiu Wu
  • Kefeng Wu
  • Tongwu Zhou
  • Long Bao
  • Jiayuan Huang
  • Wende Li
چکیده

A native polysaccharides (RRP) was isolated from Radix Rehmanniae Preparata by microwave-assisted extraction (MAE). In the method, the optimized conditions were: the ratio of raw material to water was 1:50 (g/ml); microwave irradiation power was 550W; extraction temperature was 60°C; extraction time was 30min. As a result, the average yield of RRP using MAE was 9.35%, and R.S.D. was 2.15%. Furthermore, RRP which was extracted by MAE and was purified (RRPMAE) and (PRP HWE), PRP HWE can stimulate the proliferation and differentiation of osteoblasts in vitro.

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تاریخ انتشار 2014